Does a function from $\mathbb{R}^2$ to $\mathbb{R}$ which sums to 0 on the corners of any unit square have to vanish everywhere?
I think the answer is yes but I am not sure how to prove it.
If we allow any square of any size the answer is yes - an old Putnam problem. You obtain the same result if you restrict to squares of any size parallel to the axes. However, if you take only unit squares parallel to the axes the answer is no.
Note that an obvious idea for a counterexample is to try to colour the plane with 2 colours so that any two points a unit distance apart have different colours. However this is trivial to disprove.