To construct equations from column values from your datafile, gnuplot provides a parenthesis grouping, e.g. (your equation here). In order to define your equation within parenthesis, you refer to the column value wanted by prefixing the column number with a '$' (e.g. $2 refers to the value from column 2, $3 refers to the value from column 3, etc..) and you can use those references as many times as needed within the parenthesis and each use will be replaced by the value from the numbered column.
In your case to have the 1st column be your independent x-values and your equation result the dependent value drawing the numbers from columns 2 & 3, you can do:
plot "somefile.dat" using 1:(sqrt($2*$2+$3*$3))
A short example with the input file as:
$ cat somefile.dat
1 1 1
2 2 2
3 3 3
4 4 4
5 5 5
6 6 6
7 7 7
8 8 8
9 9 9
10 10 10
Creating a short plot file for convenience:
$ cat some.plt
plot "somefile.dat" using 1:(sqrt($2*$2+$3*$3))
You can generate your plot with
$ gnuplot -p some.plt

Look things over and let me know if this is what you needed.
plot "somefile.dat" using 1:(sqrt($2*$2+$3*$3))(yes, that will do it) Inside(....)you refer to the column numbers with'$'allowing you to construct your equation.